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practical bacteriology from scratch
What you'll learn
principals of lab safety using lab equipment specially microscope
sterilization method in lab principals how to apply
bacterial culture methods bacterial stain methods
biochemical test test results
practical bacteriology from scratch
your safety first take care when working with pathogenic microorganisms wear coat when you are in lab and remove after finish keep the lab disk for equipment only wear gloves when dealing with microorganisms clean clean table before and after test mask eye protect keep your hand away from mouth and eye and nose use mechanical pipetting report the spill and disinfect the area well wash hands after any procedure deal well with fire smooth small one with towel large one use expenditure follow the instructions included in lab safety manual be professional in handling microscope there are many types with many uses bright field microscope dark field microscope phase contrast microscope fluorescent microscope electro microscope it is essential tool practice using the microscope with low power medium power high power and take care when use oil immersion lens sterilization the most important topic in microbiology without keeping your environment sterile it is impossible to obtain Apure culture sterilization methods heat sterilization sterilization by radiation sterilization by disinfectant chemicals use of antimicrobial bacterial culture is a method for isolate bacteria the culture may be natural in origin or synthetic or both culture may be ordinary enriched blood agar is enriched media They contain substances Blood ,Serum. Extract of plants or animal tissues that enhance growth of bacteria Heated blood agar chocolate agar ▪ heated blood agar chocolate agar heating blood ▪ in water path at 75c for 10 minutes ▪ Until blood become chocolate brown in color differential or specific and see it in colonies which in appearance characteristic after culture we need to sea the bacterial cell clearly stain our tool to change the background and sea bacterial morphology and enhance the contrast we sea details differential or structural preparing the smear and fix on Bunsen flame simple stain like methylene blue crystal violet negative staining is simplest method differential stain differentiate the bacteria into two groups like gram stain differentiate bacteria into gram +ve gram -ve it is invented by hans gram he use methylene blue as basic stain iodine as bacteria are not decolorized and remain violet after decolorization carbol f...